Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. 0% ± 0. VMax . Lucid Scientific. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. We would like to show you a description here but the site won’t allow us. 2. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. ~ Bio-Tek ELx808 Microplate Reader $2,795. 96- or 384-well microplate compatibility. . BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. Optimized for fluorescence microplate readers. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). #E010) Pyroplate 96-well microplate (ACC cat. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Instrument pricing. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. 5 ng per well. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Figure 1-2 Match chamfered corners of slide and adapter 2. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. This can increase throughput and efficiency, thus saving time and space in the lab. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. We would like to show you a description here but the site won’t allow us. FlexIII. 001 ABS (displayed), 0. This can increase throughput and efficiency, thus saving time and space in the lab. x. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). 2. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Microplate Reader. 5 second read per plate. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. Click Add to create the plate. x. T = I/I 0 and %T = 100 (T). Molecular Devices SpectraMax Plus 384 Absorbance. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. REF (SpectraMax. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. No. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. x. C. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. Published Dec 16, 2020 | Updated Dec 16,. 5% at 1. 012 fmol 0. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Next-generation luminescence plate reader. com. x. 1. $488. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. , Corning, NY) or Greiner. Microplate Readers. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. x. The variations include adjustable. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Compare this item. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. x. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. $3,450. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Learn more. Recertification service. Page 1 of 8 . Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. The VICTOR Nivo. Well scanning With well scanning, multiple points within each well can be read, providing a high. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 01. Plus 384. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. A = 2 - log 10 (%T). Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Please refer to the Obsolete Microplate Reader Models for more information. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. From the dropdown Menu, select Wellplates. 00. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. #N207) LAL Reagent Water (Lonza cat. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Users can choose to process all plates within the magazine or select a specific number. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 000 ABS • Resolution: 0. Unparalleled performance on a personalized platform. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. flat-bottom: 50-60 µl. Compare this item. Or scan up to 384 wells at any time to determine the best assay conditions. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 96-, 384-, and 1536-well microplates. #CA961) Methods. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 313 reference gain check fail. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. the use of a reader calibrated with primary NIST and NMI standards. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Z. HeLa. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 5 fg per well to 0. Description. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. These robust readers have been placed in labs from Antarctica to the. An industry-renowned instrument for monitoring GPCRs and ion channels. 2% in. Tristar 3 Multimode Microplate Reader. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. $3,207. Unparalleled performance on a personalized platform. 0, or. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. perfect for kinetic readings involving growth curves at 5 seconds per plate. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. #E010) Pyroplate 96-well microplate (ACC cat. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Fluorescence Microplate Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. 1% in white 96- and < 0. It has a 340-750nm Spectral Range. Materials. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. 5L) MultiWash+. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. 96-, 384-, and 1536-well microplates. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Molecular Devices' microplate readers. 20 µL (96-well plates) or 10 µL (384. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 00 USD. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. White 96-well platePlate formats. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. 0% ± 0. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. x. 000 – 4. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 0200-6262. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Infinite possibilities. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. Mode Plate Reader. the Molecular Devices SPECTRAmax® 190 and 250. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. These robust readers have been placed in labs from Antarctica to. Turn on Plate Reader and wait for it to complete its start-up routine. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. 96 well plate injection time with SpectraMax® L plate reader. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Find our technical support information along with contact details. It also integrates with the powerful SoftMax Pro Software for. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Published Dec 16,. Selects either 96-well or 384-well plate for a stand-alone read. 2b,c. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. Reads various kinds of 96-wells plates, and is equipped with shaking function. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Reader Documentation Version No. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 25, 3. 1 – 2. NanoQuant plate. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 9. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. SoftMax Pro GxP Software version 5. ©2022 Molecular Devices - All rights. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. validation, maintenance, and repair documentation. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Specifications subject to change without notice. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 5 fg per well to 0. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Instrument settings can. 000 to 2. S. $542. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Making it possible to perform kinetic analysis of solid. Quantitative, whole well cell confluence for every well. Incubation. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. the Gemini XPS reader. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 306 plate air cal. (Molecular Devices cat. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. UV Plate 96 Well, Flat Bottom, Cs 50. In SoftMax Pro, Dual Read mode was selected. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Cell viability and cytoxicity assays. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. #CA961) Methods. Order Now. Optional 16-way manifold. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Used. #23225). 030 fmol Fluorescence Polarization (FP). May require LS-50B…. Get Quote . Molecular Devices (UK) Ltd. They are also sealable using different types of adhesive mats foils or films. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Loading Buffer was also added to a set of cell-free wells for background controls. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Supplier: Molecular Devices. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. The. and the A1 corner of the plate adapter (Figure 1-2). Supersedes 1. 2. These microplates have a corner notch for use in standard plate readers. 00 . These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Plate and Setup Rack Options Screens with selection of up to four Methods shown. x. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Select up to 3 products. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The Versamax Microplate Reader is built around a grating monochromator. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 5°C at 37°C. President of Molecular Devices. The picked. 00. Inconsistent results: cannot determine whole well confluence – well after well. #SpectraMax L Config) Methods. Figure 3. UV Plate 96 Well, Flat Bottom, Cs 50. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. p-Nitroaniline - Catalog No. Published Dec 16, 2020 | Updated Dec 16, 2020. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 046 to 0. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. QC tools and IQOQ services. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 2. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. • Easy and gentle immobilization of biotin. • 0. No accessories are needed and SoftMax® Pro Software. µl) was added to each well and incubated at 37°C for 1 h. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. as well as all other Molecular Devices' microplate readers. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. Including injection time, it takes approximately 68 secs. . Two high-energy xenon flash lamps: Reading capabilities. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read.